The test strip consists of two immobilized lines printed onto nitrocellulose. The test uses an antibody sandwich test system. The first line is the capture or test line, consisting of monoclonal anti -alpha antibody. The control line is composed of goat anti-mouse IgG. The conjugate pad consists of the complementary antibody in the respective sandwiches, namely, an antibeta hCG. This antibody is conjugated to a homogenous gold colloid. During the test, capillary action draws the sample through the conjugate
pad where it binds to the immobilized capture antibody. The development of a pink line indicates presence of hCG and thus is suggestive of pregnancy, while only a control line demonstrates that the test procedure
was completed successfully, and validates the test reagents.